Heparin catcher -typical assays for measuring Heparin (UHF) in saline buffer

HEPARIN CATCHER

Background

Heparin is one of the most intensively studied glycosaminoglycans as a result of its anticoagulant properties.

Heparin is used as an anticoagulant either in its native unfractionated form (UFH) MW ~ 16 kD or as partially depolymerized form called low molecular weight ( LMW ) heparin  MW ~ 4-8 kD.

 

Heparin assay

Biomat developed three Heparin Catcher plates, as special surfaces onto which heparin at different ranges of U/ml can be immobilized.

The proposed assays exploit the different Biomat heparin catcher plates, by  quantitative enzyme-linked assays for the in vitro measurement of unfractionated heparin in low protein content fluid such as a buffer.

These heparin ELISA tests are competitive assays in which the colorimetric signal is inversely proportional to the amount of heparin present in the sample.

 

Principle of the assay

Samples to be assayed are at first mixed with a known amount of biotinylated heparin within the wells of Biomat heparin catcher plate .

The Heparin in the sample competes with biotinylated heparin to bind to the binding sites of heparin catcher plate.

After the removal of the unbound reagent and sample, a streptavidin-peroxidase conjugate is added to reveal the reaction.

The concentration in the sample is determined using a standard curve of known amounts of heparin.

 

Typical assay suitable for measuring Heparin (UHF ) in saline buffer,

in the range 0.01 to 2.0 U/ml   

in the range 0.5 to 40.0 U/ml   

in the range 2.0 to 160.0 U/ml   

(see Technical Notes n.30)

 

 

Lots are tested and certified for

·          Uniformity

·          Binding specificity

·          Reproducibility