Protein A Coated Surfaces

The Biomat product is a 96 well coated microplate with recombinant Protein A and a protein to block non-specific binding sites and to maintain stable activity.

Protein A specifically binds the Fc region of immunoglobulins of many mammalian species ( see table 1 ), with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated onto microplates, the Protein A can securely capture IgG applied directly or as antigen/antibody complexes.

Example of applications:

Product specifications

Components
Individually pouched 96-well microplates, configured in 12 removable 8-well strips or solid plate

Coating
Recombinant Protein A ( mol. weight 38.9 kDa ) ,from Staphylococcus aureus subsp. Aureus, expressed in E.coli, is coated using 200 μl/well. The strips are post-coated ( blocked ) for low non specific binding and long-term stability.

Binding capacity

The Biomat Protein A microplate shows a nominal binding capacity of ~ 5 pmol IgG/well

Sensitivity

The Biomat Protein A microplate shows a sensitivity of 0.113 ng/well of human IgG.

Uniformity
Microplates show a CV% less than 5 when used as a catcher of biotinylated human IgG in an ELISA format usingstreptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The microplates, under the indicated storage conditions 2-8 °C, are stable until the expiration date printed on the label.If opened,store in closed pouch with dessicant and use within the expiration date.

for more details see TECHNICAL NOTES N. 14

 

96 well microplates

Material: Polystyrene
Quantity per pack: 1 - Quantity per case: 50 or 100

1x8 flat bottom wells strips on 12 x 8 frame

1x8 flat bottom wells breakable strips on single well holding frame

96 wells plate - flat bottom



12x75 mm Tubes

Material: Polystyrene
Quantity per pack: 20 - Quantity per case: 1000 - 2000

Color Code
Clear TS 127506-PAR
Clear PCR06-PA/100