Reagents

Biomat provides all the components you need to build and carry out a better ELISA.

Biomat’s coating buffer, blocking solution, sample diluent, conjugate diluent and stabilizer, wash buffer, substrate and stop solution all work together to generate a very clean signal.

These products work together to address common issues during ELISA development, such as specificity, sensitivity, reproducibility and shelf-life.

Biomat’s ultimate goal is to help you develop optimized ELISAs that have a high specific signal and low background noise.

If you need assistance in your assay development or are looking for assay development services, please contact us.

Product Categories :

1)   COATING BUFFERS

               Phosphate buffered  (PB) is a balanced salt solution and is one of the buffers most commonly used for adsorptive immobilization of                  proteins and antibodies on plastic surfaces or other proteins surface for ELISA, EIA, RIA, proteins array and immuno-PCR                                  applications.

               0.1 M Phosphate Buffer pH 6.0

This buffer, at pH 6.0, is specific for proteins or antibodies that prefer a slightly acidic environment; it’s also suitable for cell culture and molecular biology applications. It helps to maintaining pH and provides phosphate ion.

               0.1 M Phosphate Buffer pH 7.2  

It’s a buffer isotonic and non-toxic to cells and has the ability to maintain osmolarity and for this is used for cells culture applications (for example washing cells, transporting cells or tissue samples, diluting cells for counting and preparing reagents). It is also often used for sample dilution in molecular biology and as protein diluent in Western blotting.

              0.1 M Carbonate/Bicarbonate Buffer pH 9.6 ( liquid )

Carbonate/Bicarbonate buffer is used for proteins and antibodies coating procedures on plastic surfaces. It’s also used for adsorptive immobilization of other protein binding surfaces for ELISA, EIA, RIA techniques, immuno-PCR and protein arrays.

 

2)   BLOCKING SOLUTIONS

The BlockerWell maintains and protects the conformation and activity of dried proteins and other biomolecules coated on plate wells to prevent degradation, denaturation and leaching, while simultaneously blocks any free binding sites left on the surface to reduce non-specific binding

BSA Blockingis a simultaneous microwell blocker and stabilizer buffer which works with both hydrophobic and hydrophilic ELISA plates. It preserves and protects biological activity of proteins and other biomolecules coated and dried on plate surface. Furthermore prevents degradation, denaturation and leaching protein and other biomolecules.

 

3)   SAMPLE DILUENT

The Sample Diluent  is a specific buffer solution for proteins or antibodies that are evaluated in the first step of a two-steps indirect  ELISA assays. The use of an appropriate antibodies/proteins diluent helps to reduce the background and minimizes non-specific binding in both ELISA and Western blotting assays.

 

4)   CONJUGATE DILUENT & STABILIZER

The  Diluent  for HRP conjugate is an useful buffer solution for proteins or antibodies HRP-conjugate, that are used in direct  or indirect ELISA assays. The use of this buffer improves the signal and does not interfere with the bonding antigen/antibody

HRP/AP Stabilizing Bufferis a universal stabilizing for dilution and stabilization of both Alkaline Phosphatase (AP)- and Horseradish Peroxidase (HRP)-conjugates.

This stabilizing buffer maintains the molecular conformation and prevents loss of activity over time; this buffer allows the conjugate to be stored at least 3 years at 2-8°C allowing of minimize assay error related to daily dilution variation

 

5)   SUBSTRATE

TMB is a one-step chromogenic substrate containing 3,3’,5,5’-tetramethylbenzidine, buffer and hydrogen peroxide.

It’s ready-to-use substrate for colorimetric detection in ELISA analysis, based on Horseradish Peroxidase labeled antibodies and proteins

 

6)   WASH BUFFER 20 x concentrate

Phosphate buffered saline (PBS)is a balanced salt solution and is one of the buffer most commonly used for washing in ELISA or Western blotting assays. It’s very important to use a good washing buffer because it is able to separate bound and unbound reagents/serum component. It’s an isotonic and non-toxic buffer to cells.

Phosphate buffered saline (PBS) solution with the detergent Tween® 20 is a balanced salt solution and is one of the buffers most commonly used as a wash solution for Western blot membranes and microplate wells ELISA assays. The Biomat wash buffer with Tween® 20 is an optimal formulation of pH stabilizer, salts and detergents designed to remove excess material from wells of microplate or membranes without disrupting the binding reaction

 

7)   STOP SOLUTION

The Stop solution is a sulphuric acid solution for colorimetric ELISA using TMB (3,3’,5,5’ - tetramethylbenzidine) substrate.

In the ELISA assays, in  presence of HRP (horseradish peroxidase) enzyme conjugates, TMB and peroxide react to produce a blue colour. The colour intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte. Addition of Stop Solution changes the colour to yellow (absorbance maximum  at 450 nm), stabilizing the colour development to enable accurate measurement of the intensity at 450 nm using a spectrophotometer or plate reader.