HB8 performances

A test simulating a competitive method shows the performance of this surface.

Principle
A limited amount of biotinylated albumin, coated on the well surface, was allowed to react with a constant amount of streptavidin peroxidase along with various amounts of unlabelled streptavidin used as standard solutions. Unbound reagents were rinsed away After incubation with TMB and stopping by adding Sulphuric Acid, the colour intensity was read at 450 nm.

Calculation of results
The enzymatic activity, present in the well, is inversely proportional to the concentration of unlabelled streptavidin present in the standard solution.

Table 1 shows the records of the absorbance at 450 nm for each point of standard solution.

The maximum binding reactivity (B Max) is represented by the absorbance derived from streptavidin-peroxidase in the presence of 0 ng of unlabelled streptavidin.

The presence of unlabelled streptavidin in the standard solutions is expressed using a percentage ratio between the relative absorbance of that standard solution (B standard concentration) and the absorbance derived from streptavidin-peroxidase in the presence of 0 ng of unlabelled streptavidin.